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raybio ® human apoptosis antibody array kit (RayBiotech inc)

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RayBiotech inc raybio ® human apoptosis antibody array kit

Boldine induces <t>apoptosis</t> in MDA-MB-231 cancer cells. ( A ) Untreated MDA-MB-231 cells demonstrated normal cellular structure. Early apoptosis features, including membrane blebbing and chromatin condensation, were observed after ( B ) 24 and ( C ) 48 hours of treatment with boldine. ( D ) Late apoptosis and necrosis were noticed after 72 hours of treatment with boldine (magnification, 200×).

Raybio ® Human Apoptosis Antibody Array Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raybio+human+apoptosis+antibody+array+kit/raybio+human+apoptosis+antibody+array+kit/pmc04057328-96-27-26
Average 90 stars, based on 1 article reviews

raybio ® human apoptosis antibody array kit - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models"

Article Title: Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S58178

Boldine induces apoptosis in MDA-MB-231 cancer cells. ( A ) Untreated MDA-MB-231 cells demonstrated normal cellular structure. Early apoptosis features, including membrane blebbing and chromatin condensation, were observed after ( B ) 24 and ( C ) 48 hours of treatment with boldine. ( D ) Late apoptosis and necrosis were noticed after 72 hours of treatment with boldine (magnification, 200×).

Figure Legend Snippet: Boldine induces apoptosis in MDA-MB-231 cancer cells. ( A ) Untreated MDA-MB-231 cells demonstrated normal cellular structure. Early apoptosis features, including membrane blebbing and chromatin condensation, were observed after ( B ) 24 and ( C ) 48 hours of treatment with boldine. ( D ) Late apoptosis and necrosis were noticed after 72 hours of treatment with boldine (magnification, 200×).

Techniques Used:

Flow cytometry and DNA ladder analysis of boldine-treated MDA-MB-231 cancer cells. ( A ) Apoptosis assay by flow cytometry analysis. MDA-MB-231 cells treated with different concentrations of boldine were collected and stained with Annexin V and propidium iodide followed by flow cytometry analysis. Numbers shown are percentages of cells in each quadrant. ( B ) Electrophoresis separation of isolated total DNA from control and boldine-treated MDA-MB-231 cells after 24 hours. Abbreviation: DNA, deoxyribonucleic acid; 100 bp, DNA ladder marker; −ve ctrl, negative control.

Figure Legend Snippet: Flow cytometry and DNA ladder analysis of boldine-treated MDA-MB-231 cancer cells. ( A ) Apoptosis assay by flow cytometry analysis. MDA-MB-231 cells treated with different concentrations of boldine were collected and stained with Annexin V and propidium iodide followed by flow cytometry analysis. Numbers shown are percentages of cells in each quadrant. ( B ) Electrophoresis separation of isolated total DNA from control and boldine-treated MDA-MB-231 cells after 24 hours. Abbreviation: DNA, deoxyribonucleic acid; 100 bp, DNA ladder marker; −ve ctrl, negative control.

Techniques Used: Flow Cytometry, Apoptosis Assay, Staining, Electrophoresis, Isolation, Marker, Negative Control

Effect of boldine on expression of apoptosis-related molecules. Human apoptosis proteome profiler array in MDA-MB-231 cells treated with boldine at an IC 50 dose for 24 hours. Graph shows the fold change difference between treated and untreated control cells. The results are represented as the mean ± standard deviation of three independent experiments.

Figure Legend Snippet: Effect of boldine on expression of apoptosis-related molecules. Human apoptosis proteome profiler array in MDA-MB-231 cells treated with boldine at an IC 50 dose for 24 hours. Graph shows the fold change difference between treated and untreated control cells. The results are represented as the mean ± standard deviation of three independent experiments.

Techniques Used: Expressing, Standard Deviation

Ab Array:

Article Title: The influence of simulated microgravity on proliferation and apoptosis in U251 glioma cells.
Article Snippet: Several studies have indicated that microgravity can influence cellular progression, proliferation, and apoptosis in tumor cell lines.. In this study, we observed that simulated microgravity inhibited proliferation and induced apoptosis in U251 malignant glioma (U251MG) cells.. Furthermore, expression of the apoptosis-associated proteins, p21 and insulin-like growth factor binding protein-2 (IGFBP-2), was upregulated and downregulated, respectively, following exposure to simulated microgravity.

Article Title: The Presence of HIV-1 Tat Protein Second Exon Delays Fas Protein-mediated Apoptosis in CD4 + T Lymphocytes
Article Snippet: .. Antibody-based Apoptosis Microarray RayBio human apoptosis antibody array kit (RayBiotech Inc., Norcross, GA) detects the relative level of 43 apoptosis-related proteins in cell lysates by using an array of antibodies spotted on a glass chip. ..

Article Title: The Overexpression of Hypomethylated miR-663 Induces Chemotherapy Resistance in Human Breast Cancer Cells by Targeting Heparin Sulfate Proteoglycan 2 (HSPG2)
Article Snippet: .. Human Apoptosis Antibody Array—We used the RayBio Human Apoptosis Antibody Array kit (RayBiotech) to simultaneously detect the relative levels of 43 apoptosis-related proteins in lysates of MDA-MB-231 cells with or without miR-663 that were treatedwith 0.25 MADM.The protein extracts were prepared, and 300 g of eachwere analyzed according to the kit manufacturer’s suggestions. ..

Article Title: A Phenylbutenoid Dimer, cis -3-(3′,4′-Dimethoxyphenyl)-4-[( E )-3′′′,4′′′-Dimethoxystyryl] Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway
Article Snippet: Cells in different treatment groups were adjusted to the same density, stained with 10 μ g/mL Rh123 in the dark followed by rinsing in PBS, and photographed under the fluorescent microscope (Olympus BX60F5, Japan). .. To determine the probable pathway of apoptosis induction mediated by ZC-B11 in CEMss cells, detection of several apoptosis-related markers was carried out using the Proteome Profiler Array (RayBio Human Apoptosis Antibody Array Kit, Raybiotech, USA) according to the manufacturer's instructions. ..

Article Title: Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro
Article Snippet: Data were captured, extracted, and analyzed with ArrayScan II Data Acquisition and Data Viewer version 3.0 (Cellomics). .. To investigate the pathways by which girinimbine induces apoptosis, we performed a determination of apoptosis-related proteins using the Proteome Profiler Array (RayBio Human Apoptosis Antibody Array Kit, RayBiotech, USA), according to manufacturer's instructions. ..

Article Title: In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii
Article Snippet: .. To investigate the signaling pathways by which girinimbine affects angiogenesis, we performed a determination of angiogenesis-related protein expression using the Proteome Profiler Array (RayBio Human Apoptosis Antibody Array Kit; RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions. ..

Article Title: New insights into the mechanisms of ram sperm protection by seminal plasma proteins.
Article Snippet: .. A RayBio Human Apoptosis Antibody Array kit (RayBiotech, Norcross, GA) was employed according to the manufacturer’s instructions. ..

Article Title: The Presence of HIV-1 Tat Protein Second Exon Delays Fas Protein-mediated Apoptosis in CD4 + T Lymphocytes
Article Snippet: .. RayBio human apoptosis antibody array kit (RayBiotech Inc., Norcross, GA) detects the relative level of 43 apoptosis-related proteins in cell lysates by using an array of antibodies spotted on a glass chip. ..

Microarray:

Multiple Displacement Amplification:

Protein-Protein interactions:

Expressing:

Image Search Results

Journal: Drug Design, Development and Therapy

Article Title: Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models

doi: 10.2147/DDDT.S58178

Figure Lengend Snippet: Boldine induces apoptosis in MDA-MB-231 cancer cells. ( A ) Untreated MDA-MB-231 cells demonstrated normal cellular structure. Early apoptosis features, including membrane blebbing and chromatin condensation, were observed after ( B ) 24 and ( C ) 48 hours of treatment with boldine. ( D ) Late apoptosis and necrosis were noticed after 72 hours of treatment with boldine (magnification, 200×).

Article Snippet: To determine the probable pathway of apoptosis induction mediated by boldine in MDA-MB-231 cells, detection of several apoptosis-related markers was done using the Proteome Profiler Array (RayBio ® Human Apoptosis Antibody Array kit; RayBiotech, Inc., Norcross, GA, USA) according to the manufacturer’s instructions.

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models

doi: 10.2147/DDDT.S58178

Figure Lengend Snippet: Flow cytometry and DNA ladder analysis of boldine-treated MDA-MB-231 cancer cells. ( A ) Apoptosis assay by flow cytometry analysis. MDA-MB-231 cells treated with different concentrations of boldine were collected and stained with Annexin V and propidium iodide followed by flow cytometry analysis. Numbers shown are percentages of cells in each quadrant. ( B ) Electrophoresis separation of isolated total DNA from control and boldine-treated MDA-MB-231 cells after 24 hours. Abbreviation: DNA, deoxyribonucleic acid; 100 bp, DNA ladder marker; −ve ctrl, negative control.

Techniques: Flow Cytometry, Apoptosis Assay, Staining, Electrophoresis, Isolation, Marker, Negative Control

Journal: Drug Design, Development and Therapy

Article Title: Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models

doi: 10.2147/DDDT.S58178

Figure Lengend Snippet: Effect of boldine on expression of apoptosis-related molecules. Human apoptosis proteome profiler array in MDA-MB-231 cells treated with boldine at an IC 50 dose for 24 hours. Graph shows the fold change difference between treated and untreated control cells. The results are represented as the mean ± standard deviation of three independent experiments.

Techniques: Expressing, Standard Deviation

Effects of girinimbine on the morphological changes of A549 cells after 24 h treatment. Apoptosis was monitored by phase contrast ((a) control, (b) treatment 9 μ M, and (c) treatment 19 μ M) and fluorescence microscopy ((d) control, (e) treatment 9 μ M, and (f) treatment 19 μ M). Arrows indicate the cell shrinkage, ruffling, and blebbing of cell membrane while arrowheads indicate the annexin V binding to phosphatidylserine. Magnification 20X.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro

doi: 10.1155/2013/689865

Figure Lengend Snippet: Effects of girinimbine on the morphological changes of A549 cells after 24 h treatment. Apoptosis was monitored by phase contrast ((a) control, (b) treatment 9 μ M, and (c) treatment 19 μ M) and fluorescence microscopy ((d) control, (e) treatment 9 μ M, and (f) treatment 19 μ M). Arrows indicate the cell shrinkage, ruffling, and blebbing of cell membrane while arrowheads indicate the annexin V binding to phosphatidylserine. Magnification 20X.

Article Snippet: To investigate the pathways by which girinimbine induces apoptosis, we performed a determination of apoptosis-related proteins using the Proteome Profiler Array (RayBio Human Apoptosis Antibody Array Kit, RayBiotech, USA), according to manufacturer's instructions.

Techniques: Fluorescence, Microscopy, Binding Assay

Effects of girinimbine on A549 cells ROS generation. (a) DCF-fluorescence intensity after ascorbic acid and 19 μ M of girinimbine exposure at 4, 8, and 24 h. Rate of apoptosis in terms of fluorescent intensity (b) and average nuclear object size (c) of nucleus of the girinimbine treatment was pretreated with 100 mM ascorbic acid. Values are mean ± SD from three independent experiments. Triplicates of each treatment group were used in each independent experiment. The statistical significance is expressed as ** P < 0.01; * P < 0.05. Fluorescent intensity units directly represent the amount of ROS production.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro

doi: 10.1155/2013/689865

Figure Lengend Snippet: Effects of girinimbine on A549 cells ROS generation. (a) DCF-fluorescence intensity after ascorbic acid and 19 μ M of girinimbine exposure at 4, 8, and 24 h. Rate of apoptosis in terms of fluorescent intensity (b) and average nuclear object size (c) of nucleus of the girinimbine treatment was pretreated with 100 mM ascorbic acid. Values are mean ± SD from three independent experiments. Triplicates of each treatment group were used in each independent experiment. The statistical significance is expressed as ** P < 0.01; * P < 0.05. Fluorescent intensity units directly represent the amount of ROS production.

Techniques: Fluorescence

Quantitative analysis of girinimbine mediated apoptosis parameter. Changes in total nuclear intensity (a), cell permeability (b), mitochondrial membrane potential (c), and cytochrome c localization (d) were measured simultaneously in A549 cells. Following treatment with girinimbine, we saw statistically significant increase in total nuclear intensity, increased cell permeability, loss of mitochondrial membrane potential, and cytochrome c release from mitochondria. Each experiment was performed at least two times. Results are expressed as the means ± SD. Statistical analysis was performed with one-way analysis of variance (ANOVA) using GraphPad Prism software (version 4.0; GraphPad Software Inc., San Diego, CA). Statistical significance is expressed as ** P < 0.01; * P < 0.05.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro

doi: 10.1155/2013/689865

Figure Lengend Snippet: Quantitative analysis of girinimbine mediated apoptosis parameter. Changes in total nuclear intensity (a), cell permeability (b), mitochondrial membrane potential (c), and cytochrome c localization (d) were measured simultaneously in A549 cells. Following treatment with girinimbine, we saw statistically significant increase in total nuclear intensity, increased cell permeability, loss of mitochondrial membrane potential, and cytochrome c release from mitochondria. Each experiment was performed at least two times. Results are expressed as the means ± SD. Statistical analysis was performed with one-way analysis of variance (ANOVA) using GraphPad Prism software (version 4.0; GraphPad Software Inc., San Diego, CA). Statistical significance is expressed as ** P < 0.01; * P < 0.05.

Techniques: Permeability, Software

CD34 + CD38 − KG1a cells were insensitive to BUS. (a) KG1a cells were stained with FITC-conjugated CD38 antibody and PE-conjugated CD34 antibody and subjected to flow cytometry to analyze the purity of the CD34 + CD38 − cells population. (b, c) KG1a cells were exposed to different concentrations of BUS for 24 or 48 h (c). MTT assay was performed (b) and apoptosis (c) was detected by annexin V/PI assay. Cells in the lower right quadrant represent early apoptosis and cells in the upper right quadrant represent late apoptosis. The graph displays the means ± SD of three independent experiments. ∗∗ P < 0.01, ∗∗∗ P < 0.001 (compared with untreated KG1a cells).

Journal: BioMed Research International

Article Title: Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells

doi: 10.1155/2015/630397

Figure Lengend Snippet: CD34 + CD38 − KG1a cells were insensitive to BUS. (a) KG1a cells were stained with FITC-conjugated CD38 antibody and PE-conjugated CD34 antibody and subjected to flow cytometry to analyze the purity of the CD34 + CD38 − cells population. (b, c) KG1a cells were exposed to different concentrations of BUS for 24 or 48 h (c). MTT assay was performed (b) and apoptosis (c) was detected by annexin V/PI assay. Cells in the lower right quadrant represent early apoptosis and cells in the upper right quadrant represent late apoptosis. The graph displays the means ± SD of three independent experiments. ∗∗ P < 0.01, ∗∗∗ P < 0.001 (compared with untreated KG1a cells).

Article Snippet: Reagents include RPMI-1640 (Hyclone, SH30809.01B), fetal bovine serum (Hyclone, SH30084.03), penicillin and streptomycin (PAA, P11-010), CUR (Sigma, 458-37-7), DMSO (Amresco, 67-68-5), BUS (Sigma, 55-98-1), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Seebio, 298-93-1), hydroxypropyl methylcellulose (Amresco, 9004-65-3), anti-CD34-PE/CD38-FITC (BD Biosciences, USA), FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, USA), CycleTEST Plus DNA Kit (BD Biosciences, USA), anti-PARP (BD, USA, 1 : 500), anti-caspase-3 (CST, USA, 1 : 5000), anti-survivin (BD, USA, 1 : 5000), ym155 (SELLECK, 781661-94-7), Human Apoptosis Antibody Array Kit (RayBio, USA), electrophoresis apparatus trophoresis (Tanon EPS200), and LI-COR Odyssey Scanner (USA).

Techniques: Staining, Flow Cytometry, MTT Assay

CUR suppressed cell growth, induced S phase arrest, and induced cell apoptosis in KG1a cells. (a) KG1a cells were treated with different concentrations of CUR for 24 or 48 h. MTT assays were performed. (b) KG1a cells were treated with different concentrations of CUR for 48 h and analyzed for DNA content by flow cytometry. (c) KG1a cells were treated with CUR and inoculated in methylcellulose for 14 days and then observed under a right microscope (magnification ×40). The graph displays means ± SD of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 (compared with control). (d) KG1a cells were treated with different concentrations of CUR for 48 h and analyzed by flow cytometry. The graph displays means ± SD of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01 (compared with control).

Journal: BioMed Research International

Article Title: Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells

doi: 10.1155/2015/630397

Figure Lengend Snippet: CUR suppressed cell growth, induced S phase arrest, and induced cell apoptosis in KG1a cells. (a) KG1a cells were treated with different concentrations of CUR for 24 or 48 h. MTT assays were performed. (b) KG1a cells were treated with different concentrations of CUR for 48 h and analyzed for DNA content by flow cytometry. (c) KG1a cells were treated with CUR and inoculated in methylcellulose for 14 days and then observed under a right microscope (magnification ×40). The graph displays means ± SD of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 (compared with control). (d) KG1a cells were treated with different concentrations of CUR for 48 h and analyzed by flow cytometry. The graph displays means ± SD of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01 (compared with control).

Techniques: Flow Cytometry, Microscopy

CUR increased BUS-induced apoptosis by downregulating procaspase-3 followed by PARP degradation in KG1a cells. (a, b) KG1a cells were treated with different concentrations of CUR or BUS alone or CUR + BUS for 48 h and analyzed by flow cytometry (a) and western blot (b). The graphs represent means ± SD of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Journal: BioMed Research International

Article Title: Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells

doi: 10.1155/2015/630397

Figure Lengend Snippet: CUR increased BUS-induced apoptosis by downregulating procaspase-3 followed by PARP degradation in KG1a cells. (a, b) KG1a cells were treated with different concentrations of CUR or BUS alone or CUR + BUS for 48 h and analyzed by flow cytometry (a) and western blot (b). The graphs represent means ± SD of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Techniques: Flow Cytometry, Western Blot

Expression of apoptosis-related proteins in various treated groups.

Journal: BioMed Research International

Article Title: Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells

doi: 10.1155/2015/630397

Figure Lengend Snippet: Expression of apoptosis-related proteins in various treated groups.

Techniques: Expressing

Suppression of survivin with YM155 could induce apoptosis and increase the sensitivity to BUS in KG1a cells. (a) KG1a cells were treated with different concentrations of YM155 for 24 and 48 h and examined by MTT assay. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 (compared with control) and # P < 0.05 (compared with 48 h group). (b) KG1a cells exposed to YM155 (2.43 ng/mL) and BUS (80 μ M) alone or CUR + BUS were analyzed by flow cytometry. The graph displays means ± SD of three independent experiments. ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Journal: BioMed Research International

Article Title: Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells

doi: 10.1155/2015/630397

Figure Lengend Snippet: Suppression of survivin with YM155 could induce apoptosis and increase the sensitivity to BUS in KG1a cells. (a) KG1a cells were treated with different concentrations of YM155 for 24 and 48 h and examined by MTT assay. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 (compared with control) and # P < 0.05 (compared with 48 h group). (b) KG1a cells exposed to YM155 (2.43 ng/mL) and BUS (80 μ M) alone or CUR + BUS were analyzed by flow cytometry. The graph displays means ± SD of three independent experiments. ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Techniques: MTT Assay, Flow Cytometry

Mechanisms of CUR-induced apoptosis and enhanced sensitivity to BUS in KG1a cells, indicating the potential role of survivin.

Journal: BioMed Research International

Article Title: Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells

doi: 10.1155/2015/630397

Figure Lengend Snippet: Mechanisms of CUR-induced apoptosis and enhanced sensitivity to BUS in KG1a cells, indicating the potential role of survivin.

Techniques:

Microscopic observation of cellular morphology using phase contrast inverted microscope of CEMss cells. Cells were treated at IC 50 Boesenbergin A in time-dependent manner. ( A ) Untreated cells showed normal structure without prominent apoptosis induction and necrosis. ( B ) Early apoptosis features were seen after 24 h representing (arrows) ( C ) Blebbing were noticed in 48 h treatment (arrows). ( D ) Increasing blebbing with chromatin condensation was seen during late apoptosis after 72 h incubation of CEMss with Boesenbergin A (arrows).

Journal: BMC Complementary and Alternative Medicine

Article Title: Induction of selective cytotoxicity and apoptosis in human T4-lymphoblastoid cell line (CEMss) by boesenbergin a isolated from boesenbergia rotunda rhizomes involves mitochondrial pathway, activation of caspase 3 and G2/M phase cell cycle arrest

doi: 10.1186/1472-6882-13-41

Figure Lengend Snippet: Microscopic observation of cellular morphology using phase contrast inverted microscope of CEMss cells. Cells were treated at IC 50 Boesenbergin A in time-dependent manner. ( A ) Untreated cells showed normal structure without prominent apoptosis induction and necrosis. ( B ) Early apoptosis features were seen after 24 h representing (arrows) ( C ) Blebbing were noticed in 48 h treatment (arrows). ( D ) Increasing blebbing with chromatin condensation was seen during late apoptosis after 72 h incubation of CEMss with Boesenbergin A (arrows).

Article Snippet: To investigate the pathways by which boesenbergin A induces apoptosis, we performed a determination of apoptosis-related proteins using the Proteome Profiler Array (RayBio® Human Apoptosis Antibody Array Kit, RayBiotech, GA, USA), according to the manufacturer’s instructions.

Techniques: Inverted Microscopy, Incubation

Confocal micrograph of acridine orange and propidium iodide double-stained CEMss cells. Cells were treated at IC 50 of Boesenbergin A at time-dependent manner. ( A ) Untreated cells showed normal structure without prominent apoptosis and necrosis. ( B ) Early apoptosis features were seen after 24 h representing intercalated acridine orange (bright green) amongst the fragmented DNA (arrows). ( C ) Blebbing and nuclear margination were noticeable after 48 h treatment (arrows). ( D ) Late apoptosis was seen after 72 h post-treatment whereby a positive staining of orange color represents hallmark of late apoptosis (arrows). Figure B Histogram representing qualitative analysis of confocal micrograph consisting of acridine orange and propidium iodide double-stained treated and untreated CEMss cells. CEMss cell death via apoptosis increased significantly (p < 0.05) in a time-dependent manner. However, no significant (p > 0.05) difference was observed in the cell count of necrosis. (EA: early apoptosis; LA: late apoptosis; SN: secondary necrosis). ‘*’ indicates significant differences from the control (p < 0.05).

Journal: BMC Complementary and Alternative Medicine

doi: 10.1186/1472-6882-13-41

Figure Lengend Snippet: Confocal micrograph of acridine orange and propidium iodide double-stained CEMss cells. Cells were treated at IC 50 of Boesenbergin A at time-dependent manner. ( A ) Untreated cells showed normal structure without prominent apoptosis and necrosis. ( B ) Early apoptosis features were seen after 24 h representing intercalated acridine orange (bright green) amongst the fragmented DNA (arrows). ( C ) Blebbing and nuclear margination were noticeable after 48 h treatment (arrows). ( D ) Late apoptosis was seen after 72 h post-treatment whereby a positive staining of orange color represents hallmark of late apoptosis (arrows). Figure B Histogram representing qualitative analysis of confocal micrograph consisting of acridine orange and propidium iodide double-stained treated and untreated CEMss cells. CEMss cell death via apoptosis increased significantly (p < 0.05) in a time-dependent manner. However, no significant (p > 0.05) difference was observed in the cell count of necrosis. (EA: early apoptosis; LA: late apoptosis; SN: secondary necrosis). ‘*’ indicates significant differences from the control (p < 0.05).

Techniques: Staining, Cell Counting

Effect of Boesenbergin A on the levels of apoptosis regulatory proteins at 3, 6, 9, and 12h with β- actin as a loading control.

Journal: BMC Complementary and Alternative Medicine

doi: 10.1186/1472-6882-13-41

Figure Lengend Snippet: Effect of Boesenbergin A on the levels of apoptosis regulatory proteins at 3, 6, 9, and 12h with β- actin as a loading control.

Techniques:

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raybio ® human apoptosis antibody array kit (RayBiotech inc)

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1) Product Images from "Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models"

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